Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: CCL2 immunohistochemical staining in TCs and ICs. Upper row, ( A ) TCs, positive (ICs, negative); ( B ) Positive ICs with >6% CCL2 positivity (TCs, negative); Lower row, ( C ) TCs and ICs, negative; All photos are at 20× magnification.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Immunohistochemical staining, Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan–Meier analysis: Association of CCL2 staining in TCs with mean OS, mean DSS, or mean RFS.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan–Meier analysis: Association between CCL2 expression in TCs and prognosis. Positive CCL2 expression in TCs was associated with a shorter mean OS ( p = 0.004), mean DSS ( p = 0.036), and mean RFS ( p = 0.047) than negative CCL2 expression.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Univariate and multivariate Cox’s regression analysis: Association of CCL2 staining in TCs with mean OS, mean DSS, or mean RFS.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan–Meier analysis: Association of CCL2 staining in ICs with mean OS, mean DSS, or mean RFS.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan-Meier analysis: Association between CCL2 expression in ICs and prognosis. Positive CCL2 expression in ICs was associated with a longer mean OS (P = 0.032), mean DSS (P = 0.001) and mean RFS (P = 0.001) than negative CCL2 expression.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Univariate and multivariate Cox’s regression analysis: Association of CCL2 staining in ICs with mean OS, mean DSS, or mean RFS.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan-Meier analysis: Association of CCL2 expression stratified by tumor stage (pT2 vs. pT3+4) in ICs and prognosis. Analysis of patients in the pT2 group revealed that positive CCL2 expression was associated with longer mean DSS ( p = 0.033) and mean RFS ( p = 0.022) than negative CCL2 expression. Comparably for patients in the pT3+4 group, CCL2 expression was associated with longer mean DSS ( p = 0.030) and mean RFS ( p = 0.034).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan-Meier analysis: Association of CCL2 expression stratified by lymph node stage; (N0 vs. N1+2) in ICs and prognosis. In the N0 group, CCL2-positive patients showed a longer mean OS ( p = 0.005), mean DSS and mean RFS (both p < 0.001) than CCL2-negative patients. However, in the N1+2 group, CCL2-positive patients had a shorter mean OS ( p = 0.001), mean DSS ( p = 0.031) and RFS ( p = 0.013).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan-Meier analysis: Association of CCL2 expression with ICs and prognosis in the patient group not treated with chemotherapy. In the chemotherapy untreated (CT–) group, CCL2 positivity was positively associated with OS ( p = 0.012), DSS ( p < 0.001) and RFS ( p < 0.001). However, there was no association between CCL2 staining and OS, DSS or RFS in the chemotherapy-treated group (CT+).

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing, Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Multivariate Cox’s regression analysis: Interactions of the parameters CCL2 staining, lymph node status, and application of chemotherapy.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Staining

Journal: Cancers

Article Title: CCL2 Expression in Tumor Cells and Tumor-Infiltrating Immune Cells Shows Divergent Prognostic Potential for Bladder Cancer Patients Depending on Lymph Node Stage

doi: 10.3390/cancers12051253

Figure Lengend Snippet: Kaplan-Meier analysis: Association of CCL2 expression in the basal subtype in ICs and prognosis. In the basal subtype, CCL2 positive staining was associated with a better mean DSS ( p = 0.032) and mean RFS ( p = 0.044) than CCL2 negative staining. However, no association between CCL2 staining and prognosis was found in the other molecular subtypes.

Article Snippet: Briefly, after heat pretreatment at 120 °C for 5 min with TE–buffer pH 9 and peroxidase blocking (Dako, Hamburg, Germany), primary antibody against CCL2 (monoclonal mouse IgG1, clone 2D8, Cat.-No. AM06749PU-N, dilution 1:15,000; Acris Antibodies, Herford, Germany) was applied for 30 min.

Techniques: Expressing, Staining, Negative Staining

Journal: International Journal of Molecular Medicine

Article Title: Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway

doi: 10.3892/ijmm.2017.3327

Figure Lengend Snippet: Effects of Lefty-1 on UUO-induced renal tubulointerstitial inflammation. (A) Overexpression of Lefty-1 suppressed inflammatory cytokine (IL-1β, IL-6 and TNF-α) and chemokine (MCP-1) expression 7 days after UUO, as determined by immunohistochemistry. Magnification, ×200; scale bar, 20 μ m (B) Reverse transcription-quantitative polymerase chain reaction. Data are expressed as the means ± standard deviation of at least three independent experiments. n=6. * P<0.001 vs. the sham control group; ** P<0.05 vs. the UUO or UUO + Ad-control-treated groups. Ad, adenovirus; H&E, hematoxylin and eosin; IL, interleukin; Lefty-1, left-right determination factor 1; MCP-1, monocyte chemotactic protein-1; TNF-α, tumor necrosis factor-α; UUO, unilateral ureteral obstruction.

Article Snippet: Interleukin (IL)-1β (cat. no. sc-7884; 1:100), IL-6 (cat. no. sc-7920; 1:200), tumor necrosis factor (TNF)-α (cat. no. sc-8301; 1:100), monocyte chemotactic protein (MCP)-1 (cat. no. sc-28879; 1:200), Smad7 (cat. no. sc-11392; 1:200), fibronectin (cat. no. sc-9068; 1:200), collagen I (A1) (cat. no. sc-28657; 1:200), cluster of differentiation (CD)68 (cat. no. sc-9139; 1:100), F4/80 (cat. no. sc-25830; 1:200) and α-smooth muscle actin (α-SMA; cat. no. sc-53015; 1:300) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Over Expression, Expressing, Immunohistochemistry, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway

doi: 10.3892/ijmm.2017.3327

Figure Lengend Snippet: Anti-inflammatory effects of Lefty-1 on the LPS (5 μ g/ml)-induced expression of inflammatory proteins in NRK-52E cells. ELISA analyses indicated that the expression levels of IL-1β, IL-6, TNF-α and MCP-1 were inhibited by Lefty-1 overexpression. Data are presented as the means ± standard deviation of at least three independent experiments. n=5. * P<0.001 vs. the control group; ** P<0.05 vs. the LPS (5 μ g/ml) or LPS + Ad-control-treated groups. Ad, adenovirus; IL, interleukin; Lefty-1, left-right determination factor 1; LPS, lipopolysaccharide; MCP-1, monocyte chemotactic protein-1; TNF-α, tumor necrosis factor-α.

Article Snippet: Interleukin (IL)-1β (cat. no. sc-7884; 1:100), IL-6 (cat. no. sc-7920; 1:200), tumor necrosis factor (TNF)-α (cat. no. sc-8301; 1:100), monocyte chemotactic protein (MCP)-1 (cat. no. sc-28879; 1:200), Smad7 (cat. no. sc-11392; 1:200), fibronectin (cat. no. sc-9068; 1:200), collagen I (A1) (cat. no. sc-28657; 1:200), cluster of differentiation (CD)68 (cat. no. sc-9139; 1:100), F4/80 (cat. no. sc-25830; 1:200) and α-smooth muscle actin (α-SMA; cat. no. sc-53015; 1:300) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Over Expression, Standard Deviation